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bioRxiv · 10.1101/382804

A model-based high throughput method for fecundity estimation in fruit fly studies.

Ng'oma, E.·King, E. G.·Middleton, K. M.
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Abstract

The ability to quantify fecundity is critically important to a wide range of experimental applications, particularly in widely-used model organisms such as Drosophila melanogaster. However, the standard method of manually counting eggs is time consuming and limits the feasibility of large-scale experiments. We develop a predictive model to automate the counting of eggs from images of eggs removed from the media surface and washed onto dark filter paper. A cross-validation approach demonstrates our method performs well, with a correlation between predicted and manually counted values of 0.88. We show how this method can be applied to a large data set where egg densities vary widely.

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Ng'oma, E., King, E. G., Middleton, K. M.. 2018-08-01. A model-based high throughput method for fecundity estimation in fruit fly studies.. https://doi.org/10.1101/382804

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