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bioRxiv · 10.1101/379644

Genome-scale sequence disruption following biolistic transformation in rice and maize

Liu, J.·Nannas, N. J.·Fu, F.-f.·Shi, J.·Aspinwall, B.·Parrott, W. A.·Dawe, R. K.
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Abstract

We biolistically transformed linear 48 kb phage lambda and two different circular plasmids into rice and maize and analyzed the results by whole genome sequencing and optical mapping. While some transgenic events showed simple insertions, others showed extreme genome damage in the form of chromosome truncations, large deletions, partial trisomy, and evidence of chromothripsis and breakage-fusion bridge cycling. Several transgenic events contained megabase-scale arrays of introduced DNA mixed with genomic fragments assembled by non-homologous or microhomology-mediated joining. Damaged regions of the genome, assayed by the presence of small fragments displaced elsewhere, were often repaired without a trace, presumably by homology-dependent repair (HDR). The results suggest a model whereby successful biolistic transformation relies on a combination of end joining to insert foreign DNA and HDR to repair collateral damage caused by the microprojectiles. The differing levels of genome damage observed among transgenic events may reflect the stage of the cell cycle and the availability of templates for HDR.

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Liu, J., Nannas, N. J., Fu, F.-f., Shi, J., Aspinwall, B., Parrott, W. A., Dawe, R. K.. 2018-07-29. Genome-scale sequence disruption following biolistic transformation in rice and maize. https://doi.org/10.1101/379644

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