Modelling cancer immunomodulation using epithelial organoid cultures

Bar-Ephraim, Y. E.; Kretzschmar, K.; Asra, P.; de Jongh, E.; Boonekamp, K. E.; Drost, J.; van Gorp, J.; Pronk, A.; Smakman, N.; Gan, I. J.; Sebestyen, Z.; Kuball, J. H.; Vries, R. G.; Clevers, H.

doi:10.1101/377655

bioRxiv · 10.1101/377655

Modelling cancer immunomodulation using epithelial organoid cultures

Bar-Ephraim, Y. E.·Kretzschmar, K.·Asra, P.·de Jongh, E.·Boonekamp, K. E.·Drost, J.·van Gorp, J.·Pronk, A.·Smakman, N.·Gan, I. J.·Sebestyen, Z.·Kuball, J. H.·Vries, R. G.·Clevers, H.

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Abstract

Here we utilize organoid technology to study immune-cancer interactions and assess immunomodulation by colorectal cancer (CRC). Transcriptional profiling and flow cytometry revealed that organoids maintain differential expression of immunomodulatory molecules present in primary tumours. Finally, we established a method to model antigen-specific epithelial cell killing and cancer immunomodulation in vitro using CRC organoids co-cultured with cytotoxic T cells (CTLs).

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Bar-Ephraim, Y. E., Kretzschmar, K., Asra, P., de Jongh, E., Boonekamp, K. E., Drost, J., van Gorp, J., Pronk, A., Smakman, N., Gan, I. J., Sebestyen, Z., Kuball, J. H., Vries, R. G., Clevers, H.. 2018-08-07. Modelling cancer immunomodulation using epithelial organoid cultures. https://doi.org/10.1101/377655

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