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bioRxiv · 10.1101/359380

Non-enzymatic roles of human RAD51 at stalled replication forks

Mason, J.·Chan, Y.-L.·Weichselbaum, R. W.·Bishop, D. K.
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Abstract

The central recombination enzyme RAD51 has been implicated in replication fork processing and restart in response to replication stress. Here, we use a separation-of-function allele of RAD51 that retains DNA binding, but not strand exchange activity, to reveal mechanistic aspects of RAD51s roles in the response to replication stress. We find that cells lacking RAD51 strand exchange activity protect replication forks from MRE11-dependent degradation, as expected from previous studies. Unexpectedly we find that RAD51s strand exchange activity is not required to convert stalled forks to a form that can be degraded by DNA2. Such conversion was shown previously to require replication fork reversal, supporting a model in which fork reversal depends on a non-enzymatic function of RAD51. We also show RAD51 promotes replication restart by both strand exchange-dependent and strand exchange-independent mechanisms.

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Mason, J., Chan, Y.-L., Weichselbaum, R. W., Bishop, D. K.. 2018-06-29. Non-enzymatic roles of human RAD51 at stalled replication forks. https://doi.org/10.1101/359380

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