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bioRxiv · 10.1101/357012

Dynamic m6A methylation facilitates mRNA triaging to stress granules

Anders, M.·Chelysheva, I.·Goebel, I.·Trenkner, T.·Zhou, J.·Mao, Y.·Verzini, S.·Qian, S.-B.·Ignatova, Z.
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Abstract

Summary blurbm6A-modification in the 5 vicinity of the coding sequence of transcripts provides a selective mechanism for triaging mRNAs to stress granules and is mediated by YTHDF3 reader protein.\n\nAbstractReversible post-transcriptional modifications on messenger RNA emerge as prevalent phenomena in RNA metabolism. The most abundant among them is N6-methyladenosine (m6A) which is pivotal for RNA metabolism and function, its role in stress response remains elusive. We have discovered that in response to oxidative stress, transcripts are additionally m6A-modified in their 5 vicinity. Distinct from that of the translationally-active mRNAs, this methylation pattern provides a selective mechanism for triaging mRNAs from the translatable pool to stress-induced stress granules. These stress-induced newly methylated sites are selectively recognized by the YTH domain family 3 (YTHDF3) reader protein, thereby revealing a new role for YTHDF3 in shaping the selectivity of stress response. Our findings describe a previously unappreciated function for RNA m6A modification in the oxidative-stress response and expand the breadth of physiological roles of m6A.

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Anders, M., Chelysheva, I., Goebel, I., Trenkner, T., Zhou, J., Mao, Y., Verzini, S., Qian, S.-B., Ignatova, Z.. 2018-06-27. Dynamic m6A methylation facilitates mRNA triaging to stress granules. https://doi.org/10.1101/357012

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