Combined quantification of intracellular (phospho-)proteins and transcriptomics from fixed single cells.

Gerlach, J. P.; van Buggenum, J. A. G.; Tanis, S. E. J.; Hogeweg, M.; Heuts, B. M. H.; Muraro, M. J.; Elze, L.; Rivello, F.; Rakszewska, A.; van Oudenaarden, A.; Huck, W. T. S.; Stunnenberg, H. G.; Mulder, K. W.

doi:10.1101/356329

bioRxiv · 10.1101/356329

Combined quantification of intracellular (phospho-)proteins and transcriptomics from fixed single cells.

Gerlach, J. P.·van Buggenum, J. A. G.·Tanis, S. E. J.·Hogeweg, M.·Heuts, B. M. H.·Muraro, M. J.·Elze, L.·Rivello, F.·Rakszewska, A.·van Oudenaarden, A.·Huck, W. T. S.·Stunnenberg, H. G.·Mulder, K. W.

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Abstract

Environmental stimuli often lead to heterogeneous cellular responses and transcriptional output. We developed single-cell RNA and Immunodetection (RAID) to allow combined analysis the transcriptome and intracellular (phospho-)proteins from fixed single cells. RAID successfully recapitulated differentiation-state changes at the protein and mRNA level in human keratinocytes. Furthermore, we show that differentiated keratinocytes that retain high phosphorylated FAK levels, a feature associated with stem cells, also express a selection of stem cell associated transcripts. Our data demonstrates that RAID allows investigation of heterogenenous cellular responses to environmental signals at the mRNA and phospho-proteome level.

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Gerlach, J. P., van Buggenum, J. A. G., Tanis, S. E. J., Hogeweg, M., Heuts, B. M. H., Muraro, M. J., Elze, L., Rivello, F., Rakszewska, A., van Oudenaarden, A., Huck, W. T. S., Stunnenberg, H. G., Mulder, K. W.. 2018-06-27. Combined quantification of intracellular (phospho-)proteins and transcriptomics from fixed single cells.. https://doi.org/10.1101/356329

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