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bioRxiv · 10.1101/355719

Free-running enzymatic oligonucleotide synthesis for data storage applications

Jensen, M. A.·Griffin, P.·Davis, R. W.
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Abstract

Here we present preliminary results for a method of oligodeoxynucleotide synthesis using terminal deoxynucleotidyl transferase (TdT) to generate mixed base homopolymer runs of defined sequence. In a process we have termed Free-Running Synthesis (FRS), we allow TdT to freely add multiple bases of a given monomer. With this method, homopolymeric runs can be used for writing DNA in a data storage capacity, where a stretch of As, for example, will be read as a single base, and the transitions between successive homopolymer runs encodes information. As a proof-of-concept, we demonstrate homopolymer additions of A, C and T onto the 3 end of a 22 base initiator strand.

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Jensen, M. A., Griffin, P., Davis, R. W.. 2018-06-30. Free-running enzymatic oligonucleotide synthesis for data storage applications. https://doi.org/10.1101/355719

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