An Optically Decodable Bead Array for Linking Imaging and Sequencing with Single-Cell Resolution

Yuan, J.; Sheng, J.; Sims, P. A.

doi:10.1101/355677

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bioRxiv · 10.1101/355677

An Optically Decodable Bead Array for Linking Imaging and Sequencing with Single-Cell Resolution

Yuan, J.·Sheng, J.·Sims, P. A.

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Abstract

Optically decodable beads link the identity of an analyte or sample to a measurement through an optical barcode, enabling libraries of biomolecules to be captured on beads in solution and decoded by fluorescence. This approach has been foundational to microarray, sequencing, and flow-based expression profiling technologies. We have combined microfluidics with optically decodable beads to link phenotypic analysis of living cells to sequencing. As a proof-of-concept, we applied this to demonstrate an accurate and scalable tool for connecting live cell imaging to single-cell RNA-Seq called Single Cell Optical Phenotyping and Expression (SCOPE-Seq).

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Yuan, J., Sheng, J., Sims, P. A.. 2018-06-25. An Optically Decodable Bead Array for Linking Imaging and Sequencing with Single-Cell Resolution. https://doi.org/10.1101/355677

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