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bioRxiv · 10.1101/348151

The effect of a free radical scavenger on oxidation stress in partial bladder outlet obstruction and its relief in a rat model

Choo, M. S.·Piao, S.·Oh, S.-J.
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Abstract

AIMSTo investigate the effect of a free radical scavenger (tempol) after relief of partial bladder outlet obstruction (pBOO) on bladder function in a rat model.\n\nMETHODSpBOO was induced in 50 eight-week-old female Sprague-Dawley rats and relieved 3 weeks later. The rats were divided randomly into 5 groups: sham-operated, tempol-treated for 1 week (Treat-1w) or 3 weeks (Treat-3w), and no treatment for 1 week (nonTreat-1w) or 3 weeks (nonTreat-3w). Awaken cystometrograms were obtained 1 or 3 weeks after relief according to the grouping. The bladders were isolated and weighed. H&E, Massons trichrome and TUNEL staining were used to analyze histological changes. The oxidative stress assessed using malondialdehyde. The expression of beta-3 adrenoreceptor was examined by Western blotting.\n\nRESULTSThe tempol-treated groups exhibited a significant decrease in the number of IDCs per voiding cycle (nonTreat-1w vs. Treat-1w, 1.18{+/-}0.82 vs. 0.36{+/-}0.40, P=0.010; nonTreat-3w vs. Treat-3w, 1.51{+/-}0.69 vs. 0.23{+/-}0.25, P=0.002). The thickness and collagen fiber deposition of the detrusor muscle layer was significantly decreased in the treated groups. Apoptosis detected was mainly observed in the urothelial cell layer, although the rate of apoptosis was significantly decreased in the treated groups (48.9{+/-}3.36% vs. 32.7{+/-}11.10%, P=0.024; 25.8{+/-}4.67% vs. 15.7{+/-}9.83%, P=0.314). The tempol-treated groups showed significant decreases in the MDA concentrations at both 1 and 3 weeks after relief. The expression of the beta-3 adrenoreceptor was increased in the tempol-treated rats.\n\nCONCLUSIONSIschemic reperfusion injury after relief of pBOO caused histological and functional changes in the bladder. Free radical scavenger treatment prevented this oxidative stress.

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Choo, M. S., Piao, S., Oh, S.-J.. 2018-06-15. The effect of a free radical scavenger on oxidation stress in partial bladder outlet obstruction and its relief in a rat model. https://doi.org/10.1101/348151

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