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bioRxiv · 10.1101/347351

In silico identification of GPI-anchored proteins in Paracoccidioides

Basso, L. R.·Goncales, R. A.·Vasconcelos, E. J. R.·Reis, T. F.·Ruy, P. C.·Ruiz, J. C.·Coelho, P. S. R.
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Abstract

Glycosylphosphatidylinositol-anchored proteins (GPI-proteins) are widely found in eukaryotic organisms. In fungi, GPI-proteins are thought to be involved in diverse cellular mechanisms such as cell wall biosynthesis and cell wall remodeling, adhesion, antigenicity, and virulence. The conserved structural domains of GPI-protein allow the utilization of in silico prediction approach to identify this class of proteins using a genome-wide analysis. We used different previously characterized algorithms to search for genes that encode predicted GPI-proteins in the genome of P. brasiliensis and P. lutzii, thermal dimorphic fungi that causes paracoccidioidomycosis (PCM). By using these methods, 98 GPI-proteins were found in P. brasiliensis with orthologs in P. lutzii. A series of 28 GPI-proteins were classified in functional categories (such as glycoside hydrolases, chitin-processing proteins, and proteins involved in the biogenesis of the cell wall). Furthermore, 70 GPI-proteins exhibited homology with hypothetical conserved proteins of unknown function. These data will be an important resource for the future analysis of GPI-proteins in Paracoccidioides spp.

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Basso, L. R., Goncales, R. A., Vasconcelos, E. J. R., Reis, T. F., Ruy, P. C., Ruiz, J. C., Coelho, P. S. R.. 2018-06-15. In silico identification of GPI-anchored proteins in Paracoccidioides. https://doi.org/10.1101/347351

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