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bioRxiv · 10.1101/332395

Cytokine profile in human olfactory cleft mucus and associated changes in olfactory function

Wang, H.·Jaen, C.·Yoshikawa, K.·Haneoka, M.·Saito, N.·Nakamura, J.·Adappa, N. D.·Cohen, N. A.·Dalton, P.
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Abstract

Multiple factors, including physical changes of the nasal mucosa and epithelium and exposure to air-borne environmental agents, appear to contribute to age-related olfactory loss. However, the molecular aspects of aging-associated olfactory loss in humans are not well understood. Although inflammation can be a significant underlying cause for olfactory impairment, whether aging increases the levels of inflammatory cytokines in the human olfactory mucosa and whether any inflammatory markers are associated with age-related olfactory loss remain unclear. Using a noninvasive method for collecting human olfactory mucus, we characterized and compared inflammatory cytokines, chemokines, and some growth factors, in the mucus collected from the olfactory cleft or the anterior nasal cavity from 12 healthy, young (18-40 years old) and 12 elderly (60-85 years old) individuals. We also hoped to identify candidate molecular biomarkers associated with age-associated olfactory loss in humans. Olfactory thresholds were obtained for two odorants and individual mucus samples were analyzed using multiplex assays for the levels of 30 cytokines. Results indicated elevated levels of certain inflammatory cytokines (IL-12, MCP-1) in olfactory mucus of the elderly, and high levels of some inflammatory factors (MCP-1, IL-8, IL-13 and VEGF) were associated with reduced olfactory sensitivity, suggesting that inflammation may play a role in olfactory decline associated with aging.

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Wang, H., Jaen, C., Yoshikawa, K., Haneoka, M., Saito, N., Nakamura, J., Adappa, N. D., Cohen, N. A., Dalton, P.. 2018-05-28. Cytokine profile in human olfactory cleft mucus and associated changes in olfactory function. https://doi.org/10.1101/332395

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