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bioRxiv · 10.1101/328328

Simultaneous multiplexed amplicon sequencing and transcriptome profiling in single cells

Saikia, M.·Burnham, P.·Keshavjee, S. H.·Wang, M. F. Z.·Moral-Lopez, P.·Hinchman, M. M.·Danko, C. G.·Parker, J. S. L.·De Vlaminck, I.
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Abstract

We describe Droplet Assisted RNA Targeting by single cell sequencing (DART-seq), a versatile technology that enables multiplexed amplicon sequencing and transcriptome profiling in single cells. We applied DART-seq to simultaneously characterize the non-A-tailed transcripts of a segmented dsRNA virus and the transcriptome of the infected cell. In addition, we used DART-seq to simultaneously determine the natively paired, variable region heavy and light chain amplicons and the transcriptome of B lymphocytes.

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Saikia, M., Burnham, P., Keshavjee, S. H., Wang, M. F. Z., Moral-Lopez, P., Hinchman, M. M., Danko, C. G., Parker, J. S. L., De Vlaminck, I.. 2018-05-22. Simultaneous multiplexed amplicon sequencing and transcriptome profiling in single cells. https://doi.org/10.1101/328328

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