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bioRxiv · 10.1101/323790

Three-photon light-sheet fluorescence microscopy

Escobet-Montalban, A.·Liu, P.·Nylk, J.·Gasparoli, F. M.·Yang, Z.·Dholakia, K.
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Abstract

We present the first demonstration of three-photon excitation light-sheet fluorescence microscopy. Light-sheet fluorescence microscopy in single- and two-photon modes has emerged as a powerful wide-field, low photo-damage technique for fast volumetric imaging of biological samples. We extend this imaging modality to the three-photon regime enhancing its penetration depth. Our present study uses a standard conventional femtosecond pulsed laser at 1000 nm wavelength for the imaging of 450 {micro}m diameter cellular spheroids. In addition, we show, experimentally and through numerical simulations, the potential advantages in three-photon light-sheet microscopy of using propagation-invariant Bessel beams in preference to Gaussian beams.

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Escobet-Montalban, A., Liu, P., Nylk, J., Gasparoli, F. M., Yang, Z., Dholakia, K.. 2018-05-16. Three-photon light-sheet fluorescence microscopy. https://doi.org/10.1101/323790

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