The molecular pathogenesis of superoxide dismutase 1-linked ALS is promoted by low oxygen tension

Keskin, I.; Forsgren, E.; Lehmann, M.; Andersen, P. M.; Brännström, T.; Lange, D. J.; Synofzik, M.; Nordström, U.; Zetterström, P.; Marklund, S. L.; Gilthorpe, J. D.

doi:10.1101/320283

bioRxiv · 10.1101/320283

The molecular pathogenesis of superoxide dismutase 1-linked ALS is promoted by low oxygen tension

Keskin, I.·Forsgren, E.·Lehmann, M.·Andersen, P. M.·Brännström, T.·Lange, D. J.·Synofzik, M.·Nordström, U.·Zetterström, P.·Marklund, S. L.·Gilthorpe, J. D.

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Abstract

Mutations that destabilize superoxide dismutase 1 (SOD1) are a cause of amyotrophic lateral sclerosis (ALS). SOD1, which is located in the reducing cytosol, contains an oxidized disulfide bond required for stability. We show that the bond is an Achilles heel of the protein because it is sensitive to the oxygen tension. Culture of ALS patient-derived fibroblasts, astrocytes and induced pluripotent stem cell-derived mixed motor neuron and astrocyte cultures (MNACs) under lowered oxygen tensions caused reductive bond cleavage and misfolding. The effects were greatest in cells expressing mutant SOD1s, but also occurred in wild type SOD1 in cultures derived from patients carrying ALS-linked mutations in C9orf72, FUS and TBK1, as well as from controls. MNACs showed a greater response than the other cell types, including enhanced SOD1 aggregation, in line with the vulnerability of the motor system. Our results show that oxygen tension is a principal determinant of SOD1 stability and shed light on how risk factors for ALS, such as aging and other conditions causing reduced vascular perfusion, could lead to disease initiation and progression.\n\nSubject categories Neuroscience; Molecular Biology of Disease

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Keskin, I., Forsgren, E., Lehmann, M., Andersen, P. M., Brännström, T., Lange, D. J., Synofzik, M., Nordström, U., Zetterström, P., Marklund, S. L., Gilthorpe, J. D.. 2018-05-11. The molecular pathogenesis of superoxide dismutase 1-linked ALS is promoted by low oxygen tension. https://doi.org/10.1101/320283

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