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bioRxiv · 10.1101/319319

Tissue-specific BMAL1 cistromes reveal that enhancer-enhancer interactions regulate rhythmic transcription

Abstract

AbtsractThe mammalian circadian clock relies on the transcription factor CLOCK:BMAL1 to coordinate the rhythmic expression of thousands of genes. Consistent with the various biological functions under clock control, rhythmic gene expression is tissue-specific despite an identical clockwork mechanism in every cell. Here we show that BMAL1 DNA binding is largely tissue-specific, due to differences in chromatin accessibility between tissues and co-binding of tissue-specific transcription factors. Our results also indicate that BMAL1 ability to drive tissue-specific rhythmic transcription not only relies on the activity of BMAL1 cis-regulatory elements (CREs), but also on the activity of neighboring CREs. Characterization of the physical interactions between BMAL1 CREs and other CREs in the mouse liver reveals that interactions are quite stable, and that BMAL1 controls rhythmic transcription by regulating the activity of other CREs. This supports that much of BMAL1 target gene transcription depends on BMAL1 capacity to rhythmically regulate a network of enhancers.

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Menet, J. S., Beytebiere, J. R., Trott, A. J., Greenwell, B., Osborne, C. A., Vitet, H. M., Spence, J., Yoo, S.-H., Chen, Z., Takahashi, J. S., Ghaffari, N.. 2018-05-10. Tissue-specific BMAL1 cistromes reveal that enhancer-enhancer interactions regulate rhythmic transcription. https://doi.org/10.1101/319319

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