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bioRxiv · 10.1101/317529

Chronic starvation induces non-canonical pro-death stress granules

Abstract

Stress granules (SGs) assemble under stress-induced conditions that inhibit protein synthesis, including eIF2 phosphorylation, inhibition of the RNA helicase eIF4a, or inactivation of mTORC1. Classically defined SGs are composed of translation initiation factors, 40S ribosomes, RNA binding proteins, and poly(A)+ mRNAs, and as such represent an important compartment for storage of mRNAs and regulation of their translation. Emerging work on SGs indicates they may play important roles in cancer, neurodegenerative disease, and viral infection, often promoting survival. Yet much previous work on SGs formation and function has employed acute stress conditions, which may not accurately reflect the chronic stresses that manifest in human disease. We used prolonged nutrient starvation to investigate SG formation and function during chronic stress. Surprisingly, SGs that form under chronic nutrient starvation lack 40S ribosomes, do not actively exchange their constituent components with cytoplasmic pools, and promote cell death. These results imply that SG assembly and function in the context of prolonged nutrient starvation stress differ significantly from what has been described for acute stress conditions.\n\nSummary StatementThis work characterizes the mechanisms of formation of a novel type of stress granule that is induced in response to long-term starvation, and unlike previously described stress granules, functions in a pro-death capacity.

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BibTeXRIS

Reineke, L. C., Cheema, S. A., Dubrulle, J., Neilson, J. R.. 2018-05-09. Chronic starvation induces non-canonical pro-death stress granules. https://doi.org/10.1101/317529

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