Variability of core microbiota in newly diagnosed treatment-naïve paediatric Inflammatory Bowel Disease patients

de Meij, T. G. J.; de Groot, E.; Peeters, C.; de Boer, N.; Kneepkens, F.; Eck, A.; Benninga, M.; Savelkoul, P.; van Bodegraven, A.; Budding, D.

doi:10.1101/317263

bioRxiv · 10.1101/317263

Variability of core microbiota in newly diagnosed treatment-naïve paediatric Inflammatory Bowel Disease patients

de Meij, T. G. J.·de Groot, E.·Peeters, C.·de Boer, N.·Kneepkens, F.·Eck, A.·Benninga, M.·Savelkoul, P.·van Bodegraven, A.·Budding, D.

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Abstract

BACKGROUND & AIMSIntestinal microbiota is considered to play a crucial role in the aetiology of inflammatory bowel disease (IBD). We aimed to describe faecal microbiota composition and dynamics in a large cohort of children with de novo (naive) IBD, in comparison to healthy paediatric controls (HC).\n\nMETHODSIn this prospective study, performed at two tertiary centres, faecal samples from newly diagnosed, treatment-naive paediatric IBD patients were collected prior to bowel cleansing for colonoscopy (t0) and 1, 3 and 6 weeks and 3 months after initiation of therapy. The microbial profiles of Crohns disease (CD) and Ulcerative colitis (UC) patients were compared with HC and linked to therapeutic response. Microbiota composition was analysed by IS-pro technology.\n\nRESULTSMicrobial profiles of 104 new IBD-patients (63 CD, 41 UC, median age 14.0 years) were compared to 61 HC (median 7.8 years). IBD was mainly characterised by decreased abundance of Alistipes finegoldi and Alistipes putredinis, which characterize a healthy state microbial core. The classifier including these core species as predictors achieved an AUC of the ROC curve of .87. Core bacteria tended to regain abundance during treatment, but did not reach healthy levels.\n\nCONCLUSIONFaecal microbiota profiles of children with de novo CD and UC can be discriminated from HC with high accuracy, mainly driven by a decreased abundance of species shaping the microbial core in the healthy state. Paediatric IBD can therefore be characterized by decreased abundance of certain bacterial species reflecting the healthy state rather than by the introduction of pathogens.

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de Meij, T. G. J., de Groot, E., Peeters, C., de Boer, N., Kneepkens, F., Eck, A., Benninga, M., Savelkoul, P., van Bodegraven, A., Budding, D.. 2018-05-08. Variability of core microbiota in newly diagnosed treatment-naïve paediatric Inflammatory Bowel Disease patients. https://doi.org/10.1101/317263

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