Highly Multiplexed Single-Cell RNA-seq for Defining Cell Population and Transcriptional Spaces

Gehring, J.; Park, J. H.; Chen, S.; Thomson, M.; Pachter, L.

doi:10.1101/315333

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bioRxiv · 10.1101/315333

Highly Multiplexed Single-Cell RNA-seq for Defining Cell Population and Transcriptional Spaces

Gehring, J.·Park, J. H.·Chen, S.·Thomson, M.·Pachter, L.

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Abstract

We describe a universal sample multiplexing method for single-cell RNA-seq in which cells are chemically labeled with identifying DNA oligonucleotides. Analysis of a 96-plex perturbation experiment revealed changes in cell population structure and transcriptional states that cannot be discerned from bulk measurements, establishing a cost effective means to survey cell populations from large experiments and clinical samples with the depth and resolution of single-cell RNA-seq.

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Gehring, J., Park, J. H., Chen, S., Thomson, M., Pachter, L.. 2018-05-05. Highly Multiplexed Single-Cell RNA-seq for Defining Cell Population and Transcriptional Spaces. https://doi.org/10.1101/315333

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