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bioRxiv · 10.1101/310706

SMURF-seq for fast, multiplexed copy number profiling with long-read sequencers

Prabakar, R. K.·Xu, L.·Hicks, J.·Smith, A. D.
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Abstract

We present SMURF-seq, a protocol to efficiently sequence short DNA molecules on a long-read sequencer by randomly ligating them to form long molecules. Applying SMURF-seq using Oxford Nanopore Technologies MinION yields up to 30 countable fragments per read at present, which generates multiple copy number profiles in a single run at a reduced time and cost. More broadly, SMURF-seq expands the utility of long-read sequencers for read-counting applications, which do not benefit from increased read length.

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Prabakar, R. K., Xu, L., Hicks, J., Smith, A. D.. 2018-04-29. SMURF-seq for fast, multiplexed copy number profiling with long-read sequencers. https://doi.org/10.1101/310706

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