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bioRxiv · 10.1101/301523

MicroRNA-205 affects mouse granulosa cell apoptosis and estradiol synthesis by targeting CREB1

Pengju, Z.·Jun, W.·Hongyan, L.·Weixia, W.·Xiaohui, L.·Haiyan, L.·Chengcheng, T.·Xintao, L.·Yumin, Z.·Xinghong, W.
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Abstract

MicroRNAs-205 (miR-205), were reportedly to be involved in various physiological and pathological processes, but its biological function in follicular atresia remain unknown. In this study, we investigated the expression of miR-205 in mouse granulosa cells (mGCs), and explored its functions in primary mGCs using a serial of in vitro experiments. The result of qRT-PCR demonstrated that miR-205 expression was significantly increased in early atretic follicles (EAF), and progressively atretic follicles (PAF) compared to healthy follicles (HF). Our results also revealed that overexpression of miR-205 in mGCs significantly promoted apoptosis, caspas-3/9 activities, and inhibited estrogen E2 release, and cytochrome P450 family 19 subfamily A polypeptide 1 (CYP19A1, a key gene in E2 production) expression. Bioinformatics and luciferase reporter assays revealed that the gene of cyclic AMP response element (CRE)-binding protein 1 (CREB1) was a potential target of miR-205. qRT-PCR and western blot assays revealed that overexpression of miR-205 inhibited the expression of CREB1 in mGCs. Importantly, CREB1 upregulation partially rescued the effects of miR-205 on apoptosis, caspase-3/9 activities, E2 production and CYP19A1 expression in mGCs. Our results indicate that miR-205 may play an important role in ovarian follicular development and provide new insights into follicular atresia.

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Pengju, Z., Jun, W., Hongyan, L., Weixia, W., Xiaohui, L., Haiyan, L., Chengcheng, T., Xintao, L., Yumin, Z., Xinghong, W.. 2018-04-14. MicroRNA-205 affects mouse granulosa cell apoptosis and estradiol synthesis by targeting CREB1. https://doi.org/10.1101/301523

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