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bioRxiv · 10.1101/286658

Microtubule end tethering of a processive Kinesin-8 motor Kif18b is required for spindle positioning

McHugh, T.·Gluszek-Kustusz, A.·Welburn, J.
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Abstract

Mitotic spindle positioning specifies the plane of cell division during anaphase. Spindle orientation and positioning is therefore critical to ensure symmetric division in mitosis and asymmetric division during development. The control of astral microtubule length plays an essential role in positioning the spindle. Here we show using gene knockout that the Kinesin-8 Kif18b controls microtubule length to center the mitotic spindle at metaphase. Using an integrated approach, we reveal that Kif18b is a highly processive plus end-directed motor that uses a C-terminal non-motor microtubule-binding region to accumulate at growing microtubule plus ends. This region is regulated by phosphorylation to spatially control Kif18b accumulation at plus ends and is essential for Kif18b-dependent spindle positioning and regulation of microtubule length. Finally, we demonstrate that Kif18b shortens microtubules by increasing the catastrophe rate of dynamic microtubules. Overall, our work reveals that Kif18b utilizes its motile properties to reach microtubule ends where it regulates astral microtubule length to ensure spindle centering.

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McHugh, T., Gluszek-Kustusz, A., Welburn, J.. 2018-03-22. Microtubule end tethering of a processive Kinesin-8 motor Kif18b is required for spindle positioning. https://doi.org/10.1101/286658

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