Unmodified, autologous adipose-derived regenerative cells improve cardiac function, structure and revascularization in a porcine model of chronic myocardial infarction

Haenel, A.; Ghosn, M.; Karimi, T.; Vykoukal, J.; Kettlun, C.; Shah, D.; Dave, A.; Valderrabano, M.; Schulz, D.; Azares, A.; Raizner, A.; Alt, E.

doi:10.1101/286468

bioRxiv · 10.1101/286468

Unmodified, autologous adipose-derived regenerative cells improve cardiac function, structure and revascularization in a porcine model of chronic myocardial infarction

Haenel, A.·Ghosn, M.·Karimi, T.·Vykoukal, J.·Kettlun, C.·Shah, D.·Dave, A.·Valderrabano, M.·Schulz, D.·Azares, A.·Raizner, A.·Alt, E.

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Abstract

Numerous studies have investigated cell-based therapies for myocardial infarction (MI), with mixed results. In the present study the left anterior descending (LAD) artery of pigs was occluded for 180 min. Four weeks later, the mean left ventricular ejection fraction (LVEF) was shown to have been reduced to approximately 35%. At that time, 18x106 unmodified, autologous adipose-derived regenerative cells (UA-ADRCs) were delivered into the LAD vein (control: delivery of saline). Six weeks following UA-ADRCs/saline delivery, the mean LVEF had increased by 18% (p<0.01) after delivery of UA-ADRCs, but was unchanged after delivery of saline. This is among the best outcome ever reported in studies on porcine animal models of cell-based therapies for MI in which functional outcome was assessed with cardiac magnetic resonance imaging. The unique combination of the procedure used for isolating UA-ADRCs, the late cell delivery time and the uncommon cell delivery route applied in the present study may open new horizons for cell-based therapies for MI.

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Haenel, A., Ghosn, M., Karimi, T., Vykoukal, J., Kettlun, C., Shah, D., Dave, A., Valderrabano, M., Schulz, D., Azares, A., Raizner, A., Alt, E.. 2018-03-21. Unmodified, autologous adipose-derived regenerative cells improve cardiac function, structure and revascularization in a porcine model of chronic myocardial infarction. https://doi.org/10.1101/286468

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