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bioRxiv · 10.1101/279331

Effect of tributyltin chloride (TBT-Cl) exposure on expression of HSP90β1 in the river pufferfish (Takifugu obscurus): evidences for its immunologic function involving in exploring process

Xu, D.-p.·Fang, D.-a.·Zhao, C.-s.·Jiang, S.-l.·Hu, H.-Y.
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Abstract

HSP90{beta}1 (known as GP96) is a vital endoplasmic reticulum depended chaperonin among the HSPs family. It plays important roles in regulating the growth, development, differentiation, and apoptosis of cells. Furthermore, it always processes and presents antigen of the tumor and keeps balance for the intracellular environment. In the present study, we explored the effect of tributyltin chloride (TBT-Cl) exposure on HSP90{beta}1 expression in river pufferfish, Takifugu obscurus. The full length of To-HSP90{beta}1 was gained with 2775 bp in length, an ORF obtained with 2412 bp encoding an 803 aa polypeptide. The phylogenetic tree was constructed and showed the close relationship to other fish species. The HSP90{beta}1 mRNA transcript was expressed in all tissues investigated. After the acute and chronic exposure of TBT-Cl, the mRNA level of To-HSP90{beta}1 significantly up-regulated in tissues of liver and gill. Moreover, the histochemistry study indicated the injury degree of TBT-Cl on liver and gill. Immunohistochemistry (IHC) staining results implied the cytoplasm reorganization after TBT-Cl stress and the function of immunoregulation for To-HSP90{beta}1. All the results indicated that HSP90{beta}1 may involve in the resistance to the invasion of TBT-Cl for keeping autoimmune homeostasis.

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Xu, D.-p., Fang, D.-a., Zhao, C.-s., Jiang, S.-l., Hu, H.-Y.. 2018-03-09. Effect of tributyltin chloride (TBT-Cl) exposure on expression of HSP90β1 in the river pufferfish (Takifugu obscurus): evidences for its immunologic function involving in exploring process. https://doi.org/10.1101/279331

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