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bioRxiv · 10.1101/273938

High-precision CRISPR-Cas9 base editors with minimized bystander and off-target mutations

Gerhke, J. M.·Cervantes, O. R.·Clement, M. K.·Pinello, L.·Joung, J. K.
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Abstract

Recently described base editor (BE) technology, which uses CRISPR-Cas9 to direct cytidine deaminase enzymatic activity to specific genomic loci, enables the highly efficient introduction of precise cytidine-to-thymidine (C [->] T) DNA alterations in many different cell types and organisms1-6. In contrast to genome-editing nucleases7-9, BEs avoid the need to introduce double-strand breaks or exogenous donor DNA templates and induce lower levels of unwanted variable-length insertion/deletion mutations (indels)1,2,10. However, existing BEs can also efficiently create unwanted C to T alterations when more than one C is present within the five base pair \"editing window\" of these proteins, a lack of precision that can cause potentially deleterious by ...

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Gerhke, J. M., Cervantes, O. R., Clement, M. K., Pinello, L., Joung, J. K.. 2018-03-01. High-precision CRISPR-Cas9 base editors with minimized bystander and off-target mutations. https://doi.org/10.1101/273938

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