Cold shock as a screen for genes involved in cold acclimatization in Neurospora crassa

Watters, M. K.; Manzanilla, V.; Howell, H.; Mehreteab, A.; Rose, E.; Walters, N.; Seitz, N.; Nava, J.; Kekelik, S.; Knuth, L.; Scivinsky, B.

doi:10.1101/259580

bioRxiv · 10.1101/259580

Cold shock as a screen for genes involved in cold acclimatization in Neurospora crassa

Watters, M. K.·Manzanilla, V.·Howell, H.·Mehreteab, A.·Rose, E.·Walters, N.·Seitz, N.·Nava, J.·Kekelik, S.·Knuth, L.·Scivinsky, B.

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Abstract

When subjected to rapid drops of temperature (cold shock), Neurospora responds with a dramatic, but temporary shift in its branching pattern. While the cold shock response has been described morphologically, it has yet to be examined genetically. This project aims to begin the genetic characterization of the cold shock response and the associated acclimatization to cold environments. We report here the results of a screen of mutants from the Neurospora knockout library for alterations in their morphological response to cold shock and thus, their ability to acclimatize to the cold. Three groups of knockouts were selected to be subject to this screen: genes previously suspected to be involved in hyphal development as well as knockouts resulting in morphological changes; transcription factors; and genes homologous to E. coli genes known to alter their expression in response to cold shock. Several strains were identified with altered responses. The genes impacted in these mutants are listed and discussed. A significant percentage (81%) of the knockouts of genes homologous to those previously identified in E. coli showed altered cold shock responses in Neurospora - suggesting that the response in these two organisms is largely shared in common.

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Watters, M. K., Manzanilla, V., Howell, H., Mehreteab, A., Rose, E., Walters, N., Seitz, N., Nava, J., Kekelik, S., Knuth, L., Scivinsky, B.. 2018-02-04. Cold shock as a screen for genes involved in cold acclimatization in Neurospora crassa. https://doi.org/10.1101/259580

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