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bioRxiv · 10.1101/255364

Multicolor single particle reconstruction of protein complexes

Sieben, C.·Banterle, N.·Douglass, K.·Gonczy, P.·Manley, S.
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Abstract

Single-particle reconstruction (SPR) from electron microscopy images is widely used in structural biology, but lacks direct information on protein identity. To address this limitation, we developed a computational and analytical framework that reconstructs and co-aligns multiple proteins from 2D super-resolution fluorescence images. We demonstrate our method by generating multi-color 3D reconstructions of several proteins within the human centriole and procentriole, revealing their relative locations, dimensions and orientations.

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Sieben, C., Banterle, N., Douglass, K., Gonczy, P., Manley, S.. 2018-01-28. Multicolor single particle reconstruction of protein complexes. https://doi.org/10.1101/255364

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