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bioRxiv · 10.1101/243600

Modelling Signalling Networks from Perturbation Data

Dorel, M.·Klinger, B.·Sieber, A.·Prahallad, A.·Gross, T.·Bosdriesz, E.·Wessels, L.·Bluthgen, N.
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Abstract

MotivationIntracellular signalling is realized by complex signalling networks which are almost impossible to understand without network models, especially if feedbacks are involved. Modular Response Analysis (MRA) is a convenient modelling method to study signalling networks in various contexts.\n\nResultsWe developed a derivative of MRA that is suited to model signalling networks from incomplete perturbation schemes and multi-perturbation data. We applied the method to study the effect of SHP2, a protein that has been implicated in resistance to targeted therapy in colon cancer, using data from a knock out and parental colon cancer cell line. We find that SHP2 is required for MAPK signalling, whereas AKT signalling only partially depends on SHP2.\n\nAvailabilityAn R-package is available at https://github.com/MathurinD/STASNet\n\nContactnils.bluethgen@charite.de

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Dorel, M., Klinger, B., Sieber, A., Prahallad, A., Gross, T., Bosdriesz, E., Wessels, L., Bluthgen, N.. 2018-01-05. Modelling Signalling Networks from Perturbation Data. https://doi.org/10.1101/243600

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