False discovery rate estimation and heterobifunctional cross-linkers

Fischer, L.; Rappsilber, J.

doi:10.1101/239715

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bioRxiv · 10.1101/239715

False discovery rate estimation and heterobifunctional cross-linkers

Fischer, L.·Rappsilber, J.

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Abstract

False discovery rate (FDR) estimation is a cornerstone of proteomics that has recently been adapted to cross-linking/mass spectrometry. Here we demonstrate that heterobifunctional cross-linkers, while theoretically different from homobifunctional cross-linkers, need not be considered separately in practice. We develop and then evaluate the impact of applying a correct FDR formula for use of heterobifunctional cross-linkers and conclude that there are minimal practical advantages. Hence a single formula can be applied to data generated from the many different non-cleavable cross-linkers.

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Fischer, L., Rappsilber, J.. 2017-12-28. False discovery rate estimation and heterobifunctional cross-linkers. https://doi.org/10.1101/239715

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