Stability, affinity and chromatic variants of the glutamate sensor iGluSnFR

Marvin, J. S.; Scholl, B.; Wilson, D. E.; Podgorski, K.; Kazemipour, A.; Mueller, J. A.; Schoch-McGovern, S.; Wang, S. S.-H.; Urra Quiroz, F. J.; Rebola, N.; Bao, H.; Little, J. P.; Tkachuk, A. N.; Hantman, A. W.; Chapman, E. R.; Dietrich, D.; DiGregorio, D. A.; Fitzpatrick, D.; Looger, L. L.

doi:10.1101/235176

bioRxiv · 10.1101/235176

Stability, affinity and chromatic variants of the glutamate sensor iGluSnFR

Marvin, J. S.·Scholl, B.·Wilson, D. E.·Podgorski, K.·Kazemipour, A.·Mueller, J. A.·Schoch-McGovern, S.·Wang, S. S.-H.·Urra Quiroz, F. J.·Rebola, N.·Bao, H.·Little, J. P.·Tkachuk, A. N.·Hantman, A. W.·Chapman, E. R.·Dietrich, D.·DiGregorio, D. A.·Fitzpatrick, D.·Looger, L. L.

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Abstract

Single-wavelength fluorescent reporters allow visualization of specific neurotransmitters with high spatial and temporal resolution. We report variants of the glutamate sensor iGluSnFR that are functionally brighter; can detect sub-micromolar to millimolar concentrations of glutamate; and have blue, green or yellow emission profiles. These variants allow in vivo imaging where original-iGluSnFR was too dim, reveal glutamate transients at individual spine heads, and permit kilohertz imaging with inexpensive, powerful fiber lasers.

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Marvin, J. S., Scholl, B., Wilson, D. E., Podgorski, K., Kazemipour, A., Mueller, J. A., Schoch-McGovern, S., Wang, S. S.-H., Urra Quiroz, F. J., Rebola, N., Bao, H., Little, J. P., Tkachuk, A. N., Hantman, A. W., Chapman, E. R., Dietrich, D., DiGregorio, D. A., Fitzpatrick, D., Looger, L. L.. 2017-12-15. Stability, affinity and chromatic variants of the glutamate sensor iGluSnFR. https://doi.org/10.1101/235176

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