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bioRxiv · 10.1101/232728

Transcriptome Analysis of Adult C. elegans Cells Reveals Tissue-specific Gene and Isoform Expression

Kaletsky, R.·Yao, V.·Williams, A.·Runnels, A. M.·King, S. B.·Tadych, A.·Zhou, S.·Troyanskaya, O. G.·Murphy, C. T.
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Abstract

The biology and behavior of adults differ substantially from those of developing animals, and cell-specific information is critical for deciphering the biology of multicellular animals. Thus, adult tissue-specific transcriptomic data are critical for understanding molecular mechanisms that control their phenotypes. We used adult cell-specific isolation to identify the transcriptomes of C. elegans four major tissues (or \"tissue-ome\"), identifying ubiquitously expressed and tissue-specific \"super-enriched\" genes. These data newly reveal the hypodermis metabolic character, suggest potential worm-human tissue orthologies, and identify tissue-specific changes in the Insulin/IGF-1 signaling pathway. Tissue-specific alternative splicing analysis identified a large set of collagen isoforms and a neuron-specific CREB isoform. Finally, we developed a machine learning-based prediction tool for 70 sub-tissue cell types, which we used to predict cellular expression differences in IIS/FOXO signaling, stage-specific TGF-b activity, and basal vs. memory-induced CREB transcription. Together, these data provide a rich resource for understanding the biology governing multicellular adult animals

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Kaletsky, R., Yao, V., Williams, A., Runnels, A. M., King, S. B., Tadych, A., Zhou, S., Troyanskaya, O. G., Murphy, C. T.. 2017-12-12. Transcriptome Analysis of Adult C. elegans Cells Reveals Tissue-specific Gene and Isoform Expression. https://doi.org/10.1101/232728

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