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bioRxiv · 10.1101/218438

One-step generation of monoclonal B cell receptor mice capable of class switch recombination and somatic hypermutation

Jacobsen, J. T.·Mesin, L.·Markoulaki, S.·Cavazzoni, C. B.·Bousbaine, D.·Jaenisch, R.·Victora, G. D.
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Abstract

We developed a method for rapid generation of B cell receptor (BCR) monoclonal mice expressing pre-rearranged Igh and Igk chains monoallelically from the Igh locus by CRISPR/Cas9 injection into fertilized oocytes. B cells from these mice undergo somatic hypermutation (SHM), class switch recombination (CSR), and affinity-based selection in germinal centers. This method combines the practicality of BCR transgenes with the ability to study Ig SHM, CSR and affinity maturation.

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Jacobsen, J. T., Mesin, L., Markoulaki, S., Cavazzoni, C. B., Bousbaine, D., Jaenisch, R., Victora, G. D.. 2017-11-13. One-step generation of monoclonal B cell receptor mice capable of class switch recombination and somatic hypermutation. https://doi.org/10.1101/218438

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