Blue light is a universal tactic signal for Escherichia coli

Perlova, T.; Gruebele, M.; Chemla, Y. R.

doi:10.1101/211474

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bioRxiv · 10.1101/211474

Blue light is a universal tactic signal for Escherichia coli

Perlova, T.·Gruebele, M.·Chemla, Y. R.

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Abstract

Blue light has been shown to elicit a tumbling response in E. coli, a non-phototrophic bacterium. The exact mechanism of this phototactic response is still unknown, and its biological significance remains unclear. Here, we quantify phototaxis in E. coli by analyzing single-cell trajectories in populations of free-swimming bacteria before and after light exposure. Bacterial strains expressing only one type of chemoreceptor reveal that all five E. coli receptors - Aer, Tar, Tsr, Tap and Trg - are capable of mediating a response to light. In particular, light exposure elicits a running response in Tap-only strain, the opposite of the tumbling response observed for all other strains. Light therefore emerges as a universal stimulus for all E. coli chemoreceptors. We also show that blue light exposure causes a reversible decrease in swimming velocity, a proxy for proton motive force. We hypothesize that rather than sensing light directly, chemoreceptors sense light-induced perturbations in proton motive force.\n\nImportanceOur findings provide new insights on the mechanism of E. coli phototaxis, showing that all five chemoreceptor types respond to light and that their interactions play an important role in cell behavior. Our results also open up new avenues for examining and manipulating E. coli taxis. Since light is a universal stimulus, it may provide a way to quantify interactions between different types of receptors. Since light is easier to control spatially and temporally than chemicals, it may be used to study swimming behavior in complex environments. Since phototaxis can cause migration of E. coli bacteria in light gradients, light may be used to control bacterial density for studying density-dependent processes in bacteria.

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Perlova, T., Gruebele, M., Chemla, Y. R.. 2017-10-30. Blue light is a universal tactic signal for Escherichia coli. https://doi.org/10.1101/211474

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