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bioRxiv · 10.1101/209437

Preovulatory oocyte aging in mice affects fertilization rate and embryonic genome activation

Demond, H.·Dankert, D.·Gruemmer, R.·Horsthemke, B.
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Abstract

Delayed ovulation, or preovulatory aging, can seriously compromise the developmental competence of oocytes. In the present study, we have investigated the effect of preovulatory aging on preimplantation embryos. Delaying ovulation with the gonadotropin releasing hormone (GnRH) antagonist Cetrorelix led to a decline in 2-cell rate from 76 to 46%. From control mice, an average of 17 embryos per mouse was retrieved. This number decreased to a mean of 5 embryos per mouse after preovulatory aging, suggesting that fertilization is impaired by aging. For analysis of zygotic genome activation, 2-cell embryos were incubated with BrUTP, which was incorporated into nascent RNA and detected by immunohistochemistry. A 2.85-fold increase in fluorescence intensity was detected after aging, pointing to a precocious activation of the genome. A possible effect of preovulatory aging on genomic imprint maintenance was investigated at the 8-cell stage. Deep amplicon bisulfite sequencing of Igf2r, Snrpn, H19 and Pou5f1 showed no significant changes between embryos derived from preovulatory-aged oocytes and control embryos, indicating stable imprint maintenance throughout epigenetic reprogramming. We conclude that preovulatory aging of the oocyte affects fertilization and embryonic genomic activation.

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Demond, H., Dankert, D., Gruemmer, R., Horsthemke, B.. 2017-10-26. Preovulatory oocyte aging in mice affects fertilization rate and embryonic genome activation. https://doi.org/10.1101/209437

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