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bioRxiv · 10.1101/207803

High degree of virulence gene diversity in Streptococcus pyogenes isolated in Central Italy

Bencardino, D.·Di Luca, M. C.·Petrelli, D.·Prenna, M.·Vitali, L. A.
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Abstract

Globally, Streptococcus pyogenes poses a continuous burden on human health, causing both self-limiting and life-threatening diseases. Therefore, studying the profile of virulence genes and their combinations is essential to monitor the epidemiology and pathogenetic potential of this important species. Thus, the aim of this study was to analyze some genetic features of clinical strains collected in Italy in 2012.\n\nWe conducted fibronectin-collagen-T antigen (FCT) region typing and emm typing in 122 S. pyogenes strains. Furthermore, several additional virulence genes were screened by polymerase chain reaction.\n\nWe found correlations between emm types and FCT region profiles. emm1 strains were mainly associated with FCT2 and FCT6, while emm89 and emm12 strains were associated with FCT4. FCT5 was mainly represented in emm4, emm6, and emm75 strains. Noteworthy, we defined subtypes for each FCT type based on the differences in single and multiple loci compared to the reference scheme used for the classification of the FCT region. In addition, new FCT types were identified. Cluster analysis based on virulence gene profiling showed a non-random distribution within each emm type.\n\nThis study showed the high variability of S. pyogenes strains and the great diversification that this pathogen has undergone during its evolution in the human host.

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Bencardino, D., Di Luca, M. C., Petrelli, D., Prenna, M., Vitali, L. A.. 2017-10-23. High degree of virulence gene diversity in Streptococcus pyogenes isolated in Central Italy. https://doi.org/10.1101/207803

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