Simultaneous cell traction and growth measurements using light

Abstract

Understanding cell mechanotransduction is important for discerning matrix structure-cell function relationships underlying health and disease. Despite the crucial role of mechanochemical signaling in phenomena such as cell migration, proliferation, and differentiation, measuring the cell-generated forces at the interface with the extracellular matrix during these biological processes remains challenging. An ideal method would provide continuous, non-destructive images of the force field applied by cells, over broad spatial and temporal scales, while simultaneously revealing the cell biological process under investigation. Toward this goal, we present the integration of a new real-time traction stress imaging modality, Hilbert phase dynamometry (HPD), with the technique of spatial light interference microscopy (SLIM) for label free monitoring of cell growth. HPD relies on extracting the displacement field in a deformable substrate, which is chemically patterned with a fluorescent grid. The displacements introduced by the cell are captured by the phase of the periodic signal associated with the grid, borrowing concepts from holography. The displacement field is uniquely converted into forces by solving an elasticity inverse problem. Because the measurement of displacement only uses the epi-fluorescence channel of an inverted microscope, we can simultaneously achieve measurements in transmission. We performed SLIM and extracted cell mass on the same field of view in addition to the measured displacement field. We used this technique to study mesenchymal stem cells and found that cells undergoing osteogenesis and adipogenesis exerted larger and more dynamic stresses than their precursor. Our results indicate that the MSCs develop the smallest forces and growth rates. We anticipate that simultaneous cell growth and traction measurements will improve our understanding of mechanotransduction, particularly during dynamic processes where the matrix properties provide context to guide cells towards a physiological or pathological outcome, e.g., tissue morphogenesis, or cancer metastasis.