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bioRxiv · 10.1101/2025.11.12.687958

Recovery of infectious Oz virus from cloned cDNA

Rakib, T. M.·Akter, L.·Shimoda, H.·Kuroda, Y.·Matsugo, H.·Urata, S.·Matsumoto, Y.
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Abstract

Oz virus (OZV) is a tick-borne, six-segmented, negative-strand RNA virus in the genus Thogotovirus, family Orthomyxoviridae. A fatal human infection was reported in Japan in 2023. In this study, we established a reverse genetics system to generate infectious recombinant OZV. Six plasmids encoding the full-length OZV genome segments under a murine RNA polymerase I promoter, together with four plasmids expressing viral proteins essential for polymerase activity, were co-transfected into murine cells. This approach enabled efficient recovery of infectious OZV. The recovered recombinant virus exhibited replication kinetics comparable to wild-type OZV. This system provides a platform for molecular studies of OZV.

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Rakib, T. M., Akter, L., Shimoda, H., Kuroda, Y., Matsugo, H., Urata, S., Matsumoto, Y.. 2025-11-12. Recovery of infectious Oz virus from cloned cDNA. https://doi.org/10.1101/2025.11.12.687958

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