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bioRxiv · 10.1101/2025.11.11.687810

Weak evaporative cooling capacity and body size shape thermal limits in tropical montane forest birds

Pacioni, C.·Xu, R.·Apfelbeck, B.·Verbruggen, F.·Njoroge, P.·Githiru, M.·Strubbe, D.·Lens, L.
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Abstract

Climate warming and forest fragmentation threaten tropical biodiversity by altering microclimates and narrowing the range of temperatures within which species can maintain optimal physiological performance. We investigated the thermoregulatory capacities of five forest-dependent bird species from the Taita Hills (Kenya), a montane biodiversity hotspot experiencing severe habitat loss. Using respirometry, we measured resting metabolic rate (RMR) across a range of temperatures to determine thermoneutral zone (TNZ) limits, quantified evaporative water loss (EWL), evaporative heat loss (EHL), and metabolic heat production (MHP) as proxies of cooling capacity, and estimated heat tolerance limits (HTLs). Contrary to expectations of a classical TNZ pattern, RMR-temperature relationships were predominantly V-shaped, suggesting the absence, or marked narrowness, of a TNZ. In contrast, HTL clearly increased with body mass, with larger species tolerating higher temperatures. Evaporative cooling efficiency remained weak across all species (EHL/MHP < 1), indicating limited capacity to dissipate metabolic heat. Compared with global data, the Taita Hills species exhibited low critical temperatures and narrow thermal ranges, consistent with specialisation to the stable microclimates of tropical montane forests. Our findings suggest that small-bodied, forest-dependent tropical birds function within narrow thermal margins, which may make them especially vulnerable to rising temperatures and the microclimatic changes associated with climate change and forest fragmentation.

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Pacioni, C., Xu, R., Apfelbeck, B., Verbruggen, F., Njoroge, P., Githiru, M., Strubbe, D., Lens, L.. 2025-11-12. Weak evaporative cooling capacity and body size shape thermal limits in tropical montane forest birds. https://doi.org/10.1101/2025.11.11.687810

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