Unraveling IGK Locus in Dog Breeds: IMGT(R) New Insights into Canine Immunogenetics.

Kustro Garnica, T.; Papadaki, A.; Georga, M.; Zeitoun, G.; Jabado-Michaloud, J.; Folch, G.; Giudicelli, V.; Gonzales, P.; Chagas Lesbon, J. C.; Alves, T. G. L.; Fukumasu, H.; Kossida, S.

doi:10.1101/2025.11.10.687733

bioRxiv · 10.1101/2025.11.10.687733

Unraveling IGK Locus in Dog Breeds: IMGT(R) New Insights into Canine Immunogenetics.

Kustro Garnica, T.·Papadaki, A.·Georga, M.·Zeitoun, G.·Jabado-Michaloud, J.·Folch, G.·Giudicelli, V.·Gonzales, P.·Chagas Lesbon, J. C.·Alves, T. G. L.·Fukumasu, H.·Kossida, S.

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Abstract

Over millennia, the selective breeding of dogs (Canis lupus familiaris) has generated remarkable genetic diversity among breeds, highlighting the need for comprehensive genomic and immunogenetic studies. This research provides detailed immunoglobulin kappa light chain locus (IGK) analysis across multiple dog breeds. It aims to uncover breed-specific genetic variations and their implications for immunology and veterinary medicine. The primary objectives were to do the biocuration of the IGK locus in nine canine genome assemblies, investigate structural variations, polymorphisms, and gene diversity, and to enrich the IMGT(R) database with comprehensive IGK data from diverse breeds, creating a more inclusive genetic resource. Our extensive annotation of breeds, including the Bernese Mountain Dog, Boxer, Cairn Terrier, Labrador Retriever, Great Dane, Basenji, and German Shepherd, identified 40 genes and 97 alleles, revealing both conserved genes and unique variants across these breeds, with in silico validation through Sanger sequencing. Notably, we analyzed discrepancies in the first reference assembly from the Boxer breed (Canfam3.1), highlighting potential errors in assembly, challenges in gene and allele nomenclature, and a low-density region within the canine IGK locus. This study not only refines the understanding of IGK locus diversity but also contributes to the IMGT(R) databases, advancing future research on immunogenetic variability, somatic mutations, and immune response dynamics in canine health and disease.

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Kustro Garnica, T., Papadaki, A., Georga, M., Zeitoun, G., Jabado-Michaloud, J., Folch, G., Giudicelli, V., Gonzales, P., Chagas Lesbon, J. C., Alves, T. G. L., Fukumasu, H., Kossida, S.. 2025-11-12. Unraveling IGK Locus in Dog Breeds: IMGT(R) New Insights into Canine Immunogenetics.. https://doi.org/10.1101/2025.11.10.687733

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