eIF2B Selectively Anchors and Activates Mutant KRAS

Kim, H.; Diao, S.; Cho, K.-J.; Lee, H.-R.; Liu, J.; Egea, P.; Pantsar, T.; Kurki, M.; Ghaddar, N.; Wang, S.; Zou, J. Y.; Amiri, M.; Gannaban, R.; Hancock, J. F.; Rice, K. M.; Deng, Q.; Sasaki, A.; Asara, J. M.; Tripathi, B.; Lowy, D.; Lawrence, R.; Hatzoglou, M.; Azpilcueta-Nicolas, C. R.; Lumb, J.-P.; Columbus, J.; Turbyville, T. J.; Marshall, C. B.; Ikura, M.; Groves, J. T.; Sonenberg, N.; Walter, P.; Koromilas, A. E.

doi:10.1101/2025.11.10.686860

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bioRxiv · 10.1101/2025.11.10.686860

eIF2B Selectively Anchors and Activates Mutant KRAS

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Abstract

Much is known about how RAS oncoproteins regulate mRNA translation factors, but the reverse relationship, how translation factors influence RAS activity, has remained largely unexplored. At the plasma membrane (PM), Son of Sevenless (SOS) acts as the canonical guanine nucleotide exchange factor (GEF) for RAS proteins, yet mechanisms governing its specificity for individual RAS isoforms remain unknown. Here, we show that the translation initiation factor eIF2B, best known for its GEF function in translation initiation, forms a distinct complex with SOS and mutant KRAS at the PM, but not with other oncogenic RAS variants. Mechanistically, eIF2B acts as an allosteric regulator of SOS, selectively enhancing GDP-GTP exchange on mutant KRAS. This specificity arises from the translational activity of eIF2B, which upregulates glycosphingolipid (GSL) biosynthesis to remodel PM lipids and preferentially anchor mutant KRAS. Together, our results uncover an unexpected moonlighting function of eIF2B: acting both as a direct activator of SOS and as a regulator of GSL pathway that shapes the membrane landscape, both required for mutant KRAS activation. These insights redefine our understanding of eIF2B and mutant KRAS functions in cancer and have profound implications for KRAS-driven oncogenesis. Graphical Abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=161 SRC="FIGDIR/small/686860v1_ufig1.gif" ALT="Figure 1"> View larger version (21K): org.highwire.dtl.DTLVardef@1f50934org.highwire.dtl.DTLVardef@2f55b9org.highwire.dtl.DTLVardef@1a51e39org.highwire.dtl.DTLVardef@1634aac_HPS_FORMAT_FIGEXP M_FIG C_FIG O_LIeIF2B interacts with mutant KRAS and SOS at the plasma membrane (PM). C_LIO_LIThe eIF2B:SOS complex promotes the GTP-bound active state of mutant KRAS. C_LIO_LIeIF2B enhances the translation of B4GALT5 mRNA, encoding a key enzyme of glycosphingolipid (GSL) biosynthesis. C_LIO_LIUpregulation of the GSL metabolites, ganglioside GM3 and sulfatide SM4, remodels PM lipid composition to facilitate eIF2B:SOS:KRAS complex formation and mutant KRAS nanoclustering. C_LIO_LIThrough its interaction with SOS and stimulation of GSL synthesis, eIF2B selectively activates mutant KRAS at the PM among RAS isoforms. C_LIO_LIeIF2B is required for the development of mKRAS-driven lung adenocarcinoma in mice. C_LIO_LIeIF2B is a marker of poor prognosis in mutant KRAS-driven cancers. C_LI

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Kim, H., Diao, S., Cho, K.-J., Lee, H.-R., Liu, J., Egea, P., Pantsar, T., Kurki, M., Ghaddar, N., Wang, S., Zou, J. Y., Amiri, M., Gannaban, R., Hancock, J. F., Rice, K. M., Deng, Q., Sasaki, A., Asara, J. M., Tripathi, B., Lowy, D., Lawrence, R., Hatzoglou, M., Azpilcueta-Nicolas, C. R., Lumb, J.-P., Columbus, J., Turbyville, T. J., Marshall, C. B., Ikura, M., Groves, J. T., Sonenberg, N., Walter, P., Koromilas, A. E.. 2025-11-12. eIF2B Selectively Anchors and Activates Mutant KRAS. https://doi.org/10.1101/2025.11.10.686860

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