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bioRxiv · 10.1101/2025.11.08.687059

Engineering butyrate-producing Lachnospiraceae to treat metabolic disease

Abstract

Engineering native gut bacteria offers a route to persistent, programmable therapeutics, yet many dominant taxa remain genetically intractable. Lachnospiraceae are a prevalent and abundant family in the human gut microbiome, possessing metabolic functions generally associated with health1. Despite their promise as engineered live biotherapeutics, genetic manipulation of Lachnospiraceae remains challenging. Here, we develop a modular toolkit for Lachnospiraceae engineering, including constitutive and inducible expression and chromosomal integration systems. Applying this toolkit to the native commensal Coprococcus comes, we program secretion of the mammalian cytokine interleukin-22 (IL-22) in the mouse intestinal tract where it elicits ileal transcriptional responses consistent with cytokine signaling. In a mouse model of metabolic associated steatotic liver disease, IL-22-secreting C. comes improves glucose homeostasis and attenuates hepatic steatosis. This work demonstrates that a native Lachnospiraceae chassis can be genetically programmed to modulate host metabolic and immune physiology. The toolkit provides a generalizable foundation for Lachnospiraceae-derived microbiome therapeutics and for probing causal links between Lachnospiraceae gene programs and host phenotypes.

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Arnold, J., McClure, S., Glazier, J., Ahan, R. E., Shakya, S., Villegas, D., Chen, T. L., Fuerte-Stone, J., McGann, R., Mimee, M.. 2025-11-09. Engineering butyrate-producing Lachnospiraceae to treat metabolic disease. https://doi.org/10.1101/2025.11.08.687059

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