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bioRxiv · 10.1101/2025.11.07.687134

Morphological Identification of Ticks in district Swabi, Mardan, and Charsadda

Mohammad, A.·Hongsu, H.·Ali, A.·Zhao, J.·Wu, Y.·Alam, U.·Dai, J.·Zhao, C.·Zhao, T.·Hui, Z.·Zhihua, S.
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Abstract

Ticks are ectoparasitic arthropods that play a significant role in transmitting pathogens to humans and animals. This study aimed to address the research gap in understanding the morphological diversity and distribution of tick species in Swabi, Mardan, and Charsadda districts of Pakistan, where tick-borne diseases are prevalent but poorly documented. The primary objectives were to identify tick species, document their morphological characteristics, and map their distribution patterns across different habitats. Field surveys were conducted over nine months, collecting tick specimens from forests, grasslands, and residential areas. Specimens were preserved and analyzed using stereo-zoom microscopy and taxonomic keys for accurate classification. Key findings revealed a diverse tick fauna, including hard ticks (Ixodidae), with variations in body size, colour patterns, capitulum shape, and scutum features. Some species were widely distributed across all three districts, while others exhibited localized patterns. The study provides the first comprehensive morphological identification of tick species in these regions, highlighting their potential role in disease transmission. The implications of this research are significant for public health and disease control. The data can inform targeted interventions, enhance disease surveillance programs, and support the development of preventive measures to mitigate tick-borne diseases. By contributing to tick taxonomy and epidemiology, this study lays the groundwork for protecting public health in the study regions and beyond. This study addresses the lack of data on tick diversity in high-prevalence regions of Pakistan, providing critical insights for disease control and public health strategies. Limited information exists on the morphological diversity and distribution of tick species in Swabi, Mardan, and Charsadda districts. To identify tick species, document morphological characteristics, and map their distribution patterns in the study areas. Field surveys, specimen collection, stereo-zoom microscopy, and taxonomic classification were employed over nine months. Diverse tick fauna was identified, including hard ticks, with variations in morphology and distribution. Some species were widespread, while others were localized, highlighting potential disease transmission risks. The findings support targeted interventions, disease surveillance, and preventive measures, contributing to public health protection and tick-borne disease control in the region.

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Mohammad, A., Hongsu, H., Ali, A., Zhao, J., Wu, Y., Alam, U., Dai, J., Zhao, C., Zhao, T., Hui, Z., Zhihua, S.. 2025-11-08. Morphological Identification of Ticks in district Swabi, Mardan, and Charsadda. https://doi.org/10.1101/2025.11.07.687134

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