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bioRxiv · 10.1101/2025.10.28.684574

Dual-Field Interference as a Common Biomechanical Origin of Cell Division, Differentiation, and Aging

Bueno, A. D. M.
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Abstract

This work develops a biophysical theory in which a bioelectric field V (x, t) and a cortical stress field{sigma} (x, t) are weakly and reciprocally coupled via an overdamped electromechanical coupler. We show that interference between two fast latent modes produces a measurable slow beat fslow that acts as a tissue-level clock. By sampling the dynamics at "neutral moments"--recurring instants of phase symmetry--we derive a reduced even circle map in which healthy homeostasis corresponds to locking within a specific 2/21 Arnold tongue. We then introduce a coarse-grained dual-field algebra that collapses the continuum description into three effective blocks ({Gamma}, a, b) capturing net electromechanical gain and dissipation. In this algebraic picture, ionic and rheological perturbations are represented as smooth deformations of the parametrization space ({Gamma}, a, b), while the clock variables ({Omega}, fslow, K2) provide experimentally accessible coordinates on those deformations. This construction offers a concrete bridge between molecular-scale regulation and tissue-level mechanics, connecting subcellular control to the emergent seconds-minutes slow clock that constrains division geometry. Evaluating the membrane-potential profile at neutral moments defines a neutral charge-asymmetry observable {Delta}Qn that quantifies left-right voltage imbalance at the division axis and links the slow-phase map to directly measurable bioelectric patterns. The same neutral-map construction admits a rotational interpretation in terms of circle maps and slow precession of the locked orbit: small detunings{delta} from the ideal 2/21 plateau generate a hierarchy of time scales and predict a scaling law Tdev ~ 1/(fslow |{delta}|) relating the fast electromechanical beat to developmental timing. The theory yields four falsifiable predictions. (P1) Homeostatic epithelia exhibit a narrow shared slow-band peak in voltage and stress with high coherence. (P2) The effective forcing and coupling ({Omega}, K2), derived from physical parameters, reside within the 2/21 tongue while avoiding broad low-order resonances. (P3) A weak, frequencyspecific drive at fslow (phase-targeted entrainment) selectively increases coherence and reduces spindle-angle dispersion in unlocked states, providing a physical basis for bioelectric modulation of regenerative dynamics. (P4) Across conditions with comparable fslow, the number of neutral compensation cycles required to complete a phenotypic transition scales inversely with the detuning |{delta}|, linking slow precession of the neutral map to macroscopic developmental time. Ultimately, this framework treats cancer-like instability and senescence-like arrest not as independent pathologies, but as opposite failures of navigation in a single underlying electromechanical cycle, from persistent unlocking to rigid oversynchronization. HighlightsO_LIProposes an overdamped double-oscillator model of tissue electromechanics in which two fast latent modes generate a slow beat (fslow). C_LIO_LILinks the slow beat to an even circle map via neutral moments, identifying a specific 2/21 Arnold tongue that governs stable spindle orientation. C_LIO_LIIntroduces a quantitative dual-field algebra in which ionic (pump-like) and rheological (stiffness-like) perturbations act as deformations of coarse-grained blocks ({Gamma}, a, b), predicting matched shifts in {Omega} and fslow. C_LIO_LIDefines a neutral charge-asymmetry observable {Delta}Qn at neutral moments, recasting the locking scenarios (P1-P3) as constraints on left-right membrane-potential imbalance that can be computed from existing bioelectric models. C_LIO_LIRelates the slow beat to capture kinetics Tlock, linking fast carrier interference to mitotic (minute-scale) timing through progressive synchronization. C_LIO_LIDerives a scaling law (P4) in which the number of neutral compensation cycles required for a phenotypic transition scales inversely with the phase detuning |{delta}|, naturally generating a hierarchy of time scales from minutes to days. C_LIO_LIModels "proliferative unlocking" and "senescent overlock" as opposite dynamical failures of the dual-field clock (drift vs. rigidity), providing a unified view of cancer instability and aging. C_LIO_LIValidates a protocol for phase-targeted entrainment, predicting that a weak drive at fslow selectively recovers coherence and reduces spindle-angle dispersion in unlocked states. C_LI

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Bueno, A. D. M.. 2025-10-30. Dual-Field Interference as a Common Biomechanical Origin of Cell Division, Differentiation, and Aging. https://doi.org/10.1101/2025.10.28.684574

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