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bioRxiv · 10.1101/2025.10.16.682843

Loss of ciliary proteins IFT20 and IFT88 results in defective phagocytosis and metabolism in the RPE

Matthiessen, P. A.·Wagner, L. E.·Boldt, K.·Lieberwirth, I.·Glasser, G.·Nandrot, E. F.·May-Simera, H. L.
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Abstract

A major proportion of retinal disease-causing genes are related to the primary cilium, a microtubule-based signalling organelle essential for multiple developmental pathways. Previous work has shown that the primary cilium plays a crucial role in the development of the retinal pigment epithelium (RPE) affecting homeostasis and function, in particular phagocytosis. We used a cell biology approach to analyse the influence of ciliary genes on RPE phagocytosis and dissect the underlying molecular mechanisms. We found that loss of ciliary trafficking via depletion of Ift20 and Ift88 in RPE-J cells resulted in impaired phagocytosis, specifically by reducing photoreceptor outer segments binding, changes in apical membrane morphology and altered mitochondrial metabolism, whereas loss of Bbs6 showed no functionality phenotype. In addition, proteomics revealed mis-regulated pathways and targets, through which new phagocytosis-related proteins were identified. Our data highlight the role of primary cilia proteins in RPE function and metabolism, essential for visual health.

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Matthiessen, P. A., Wagner, L. E., Boldt, K., Lieberwirth, I., Glasser, G., Nandrot, E. F., May-Simera, H. L.. 2025-10-17. Loss of ciliary proteins IFT20 and IFT88 results in defective phagocytosis and metabolism in the RPE. https://doi.org/10.1101/2025.10.16.682843

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