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bioRxiv · 10.1101/2025.10.11.681788

An open CAR-T single-cell atlas to enable in-depth characterization and rational engineering of CAR-T products

Camara-Pena, S.·Rodriguez-Marquez, P.·Planell, N.·Calleja-Cervantes, M. E.·Jordana-Urriza, L.·Cinnirella, G.·Reich-Zeliger, S.·Rodriguez-Otero, P.·Tamariz, E.·Ochoa, I.·Yosef, N.·Rodriguez-Madoz, J. R.·Prosper, F.·Hernaez, M.
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Abstract

BRIEF ABSTRACTWe built a CAR-T cell functional atlas from over one million cells across 13 studies, integrating data from patients and healthy donors. The atlas captures 11 phenotypes, links infusion product composition with clinical response, and reveals sex- and age-dependent effects, metabolic signatures, and rare ICANS-associated cells. This open-access resource provides a foundation to understand CAR-T cell function and guide the design of next-generation therapies.

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Camara-Pena, S., Rodriguez-Marquez, P., Planell, N., Calleja-Cervantes, M. E., Jordana-Urriza, L., Cinnirella, G., Reich-Zeliger, S., Rodriguez-Otero, P., Tamariz, E., Ochoa, I., Yosef, N., Rodriguez-Madoz, J. R., Prosper, F., Hernaez, M.. 2025-10-13. An open CAR-T single-cell atlas to enable in-depth characterization and rational engineering of CAR-T products. https://doi.org/10.1101/2025.10.11.681788

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