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bioRxiv · 10.1101/2025.10.10.681766

Avocado supplementation mitigates hypertension and multi-organ injury in an L-NAME model of cardiovascular dysfunction

Amadi, J. A.·Aloy-Amadi, O. C.·Chukwuma, C. H.·Amadi, P.
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Abstract

BackgroundEndothelial dysfunction, hypertension, and multi-organ injury remain central drivers of cardiovascular disease. Avocado (Persea americana) is rich in monounsaturated fatty acids, phytosterols, and antioxidants, yet its integrative impact on hypertension-induced systemic injury has not been fully explored. MethodsMale Wistar rats (n = 4/group) were randomized into six groups: control, avocado, L-NAME, L-NAME+losartan+metaprolol succinate, L-NAME+avocado, and L-NAME+metaprolol succinate+avocado. Avocado pulp was incorporated into diet at 80% w/w. Endpoints included blood pressure indices, hematological parameters, liver enzymes, renal function tests, and correlation analyses of systolic-diastolic coupling. One-way ANOVA with Tukeys post hoc tests evaluated group differences, while forest plots and scatter analyses visualized treatment effects. ResultsL-NAME significantly elevated systolic blood pressure ({Delta}+18 mmHg), diastolic pressure ({Delta}+20 mmHg), and mean arterial pressure ({Delta}+17 mmHg; all p < 0.01) compared with controls. Avocado supplementation reduced these elevations by ~15-18 mmHg, restoring values near baseline. L-NAME increased platelet counts (p = 0.031) and trended toward leukocytosis, both of which were attenuated by avocado. ALT levels were higher in L-NAME rats (p = 0.044), while AST and ALP trended upward; avocado-fed groups maintained near-control enzyme levels. Renal markers were most affected: urea (+25 mg/dl) and creatinine (+0.8 mg/dl) rose significantly in L-NAME rats (p < 0.01), but were reduced by 20-30% with avocado supplementation. Electrolytes remained unchanged. Correlation analyses revealed pathological SBP-DBP coupling in L-NAME rats (r = 0.78), abolished by avocado (r = 0.00). ConclusionAvocado supplementation mitigates L-NAME-induced hypertension and systemic injury by stabilizing blood pressure, reducing thrombocytosis, and preserving hepatic and renal function. These findings support avocado as a pleiotropic nutraceutical adjunct for cardiometabolic protection. HighlightsO_LIAvocado pulp supplementation incorporated into chow (80% w/w) mitigates L-NAME-induced hypertension in rats. C_LIO_LIDietary avocado preserves hepatic and renal biochemical function while reducing thrombocytosis under cardiovascular stress. C_LIO_LIAvocado demonstrates pleiotropic nutraceutical potential, stabilizing systemic physiology beyond conventional pharmacological therapy. C_LI Graphical abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=106 SRC="FIGDIR/small/681766v1_ufig1.gif" ALT="Figure 1"> View larger version (31K): org.highwire.dtl.DTLVardef@101b300org.highwire.dtl.DTLVardef@8f0624org.highwire.dtl.DTLVardef@1dc4ab3org.highwire.dtl.DTLVardef@1ac4130_HPS_FORMAT_FIGEXP M_FIG C_FIG

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Amadi, J. A., Aloy-Amadi, O. C., Chukwuma, C. H., Amadi, P.. 2025-10-13. Avocado supplementation mitigates hypertension and multi-organ injury in an L-NAME model of cardiovascular dysfunction. https://doi.org/10.1101/2025.10.10.681766

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