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bioRxiv · 10.1101/2025.10.10.679263

Subcellular transcriptome of radial glia reveals compartmentalized control of cortical development

D'Arcy, B.·Musso, C.·Lee, C.-F.·Serdar, L.·Perez-Sanchez, S.·Fernandez, V.·Borrell, V.·Silver, D.
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Abstract

RNA localization and local translation mediate spatial and temporal control of polarized cells, including radial glial cells (RGCs) which produce and organize neurons and glia. Within RGCs, RNAs are transported long distances to basal endfeet, where they can undergo local translation. However, the subcellular composition of RGCs and function of local gene regulation remains largely unknown. Here, we discover that basal endfeet harbor a rich transcriptome including a Dynein component critical for subcellular RGC function. By purifying RGC compartments in vivo, we discover [~]3000 endfoot transcripts, including [~]800 highly enriched compared to cell bodies. Many endfoot-enriched transcripts exhibit conserved subcellular localization in neurons and glia and are associated with neurodevelopmental disease. We show that endfoot-enriched Dync1li2 regulates RGC basal morphology and subsequently interneuron organization. Finally, we develop LOCAL-KD, a CRISPR-Cas13 based method for subcellular mRNA knockdown in vivo. Leveraging this, we demonstrate that endfoot-localized Dync1li2 is critical for RGC morphology. Our study establishes experimental paradigms to understand RNA localization in the nervous system. Moreover, we discover RGCs have a vast subcellular transcriptome, revealing foundational insights into how RGCs control cortical development.

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D'Arcy, B., Musso, C., Lee, C.-F., Serdar, L., Perez-Sanchez, S., Fernandez, V., Borrell, V., Silver, D.. 2025-10-10. Subcellular transcriptome of radial glia reveals compartmentalized control of cortical development. https://doi.org/10.1101/2025.10.10.679263

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