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bioRxiv · 10.1101/2025.10.07.680721

Long-read sequencing reveals telomere inheritance patterns from human trios

Zhou, Y.·Lougheed, D.·Cheung, W.·Thiffault, I.·Pastinen, T.·Bourque, G.
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Abstract

Telomeres are essential for maintaining genomic integrity and are associated with cellular aging and disease, yet the factors influencing their inheritance across generations remain poorly understood. Leveraging PacBio HiFi long-read sequencing and 75 parent-offspring trios (n = 225) from the Genomic Answers for Kids program, we analyzed individual telomeres across chromosomes and their inheritance. Telomere length (TL) varied between chromosome arms in a way that was consistent in parents and offsprings, with average values ranging from 5000 to 8000 base pairs. Maternal and paternal TL together were a strong predictor of child TL (R2 = 0.59). Notably, using telomeric variant repeats, we developed a tool that enabled allelic tracing for 53.3% of maternally and 49.9% of paternally inherited telomeres. In the child, paternally transmitted alleles were significantly longer than age-matched maternal ones ({Delta}mean = 409 bp, p = 2.6e-05), particularly when from older parents ({Delta}mean = 698 bp, p = 8.9e-05) and at chromosome arms with shorter average TL ({Delta}mean = 752 bp, p = 1.6e-06). These findings reveal parent-of-origin effects and heritable influences on TL, providing novel insights into telomere dynamics and their potential implications in age-related disease susceptibility.

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Zhou, Y., Lougheed, D., Cheung, W., Thiffault, I., Pastinen, T., Bourque, G.. 2025-10-07. Long-read sequencing reveals telomere inheritance patterns from human trios. https://doi.org/10.1101/2025.10.07.680721

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