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bioRxiv · 10.1101/2025.10.02.679826

Atg16l1 promotes lung transplant tolerance by regulating glycolysis in macrophages

Abstract

Lung transplant survival is limited by the development of chronic lung allograft dysfunction (CLAD), a type of graft rejection that lacks effective treatments. Autophagy plays a crucial role in maintaining cellular homeostasis. In a single-nucleotide polymorphism screen, we found that lung recipients with two copies of a common hypofunctional genetic variant of autophagy-related 16-like 1 rs2241880 (ATG16L1T300A/T300A), known to deplete this protein from macrophages, were more likely to develop early CLAD. To understand this, we used a mouse orthotopic lung transplant model. Recipients encoding myeloid cell-specific deletion of Atg16l1 (Atg16l1{Delta}/{Delta}) or who harbor an engineered orthologous mutation (Atg16l1T316A/T316A) showed similar susceptibility to CLAD. Transcript profiling and mitochondrial tracking studies indicated that increased mitochondrial damage and decreased autophagic removal of mitochondria in Atg16l1-deficient macrophages were associated with heightened activation of the hypoxia-inducible factor 1 (Hif1) pathway and accumulation of glycolytic transcripts. Metabolic analysis revealed reduced oxidative phosphorylation, increased glycolytic activity, and higher IL-1{beta} expression in Atg16l1-deficient macrophages. Notably, the development of CLAD in Atg16l1{Delta}/{Delta} lung recipients could be significantly prevented by additionally deleting Hif1 in myeloid cells or by treating with the glycolysis inhibitor 2-deoxyglucose. Our results show how a common autophagy-related genetic variant disrupts macrophage metabolism and impairs lung transplant tolerance, pointing toward potential therapeutic strategies to combat CLAD.

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BibTeXRIS

Cano, M., Liao, F., Zhou, D., Chen, C., Liu, Z., Zhu, J. H., Bernadt, C., Ebenezer, R., Davis, V., Pugh, K. N., Tao, Y., Tague, L. K., Huang, H. J., Byers, D., Hachem, R., Brody, S. L., Krupnick, A. S., Kreisel, D., Gelman, A. E.. 2025-10-04. Atg16l1 promotes lung transplant tolerance by regulating glycolysis in macrophages. https://doi.org/10.1101/2025.10.02.679826

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