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bioRxiv · 10.1101/2025.09.30.679514

GOA-1 regulates spermathecal transits

Sadeghian, F.·Cram, E. J.
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Abstract

G protein signaling regulates calcium dynamics and contractility in the C. elegans spermatheca. G protein-coupled receptors activate heterotrimeric G proteins, triggering downstream cascades, including the Gs-mediated activation of adenylyl cyclase and subsequent Protein Kinase A (PKA) activation. Our previous work identified GSA-1/Gs and PKA as key modulators of Ca2+ oscillations and tissue contractility within the C. elegans spermatheca. In this study, we show that the inhibitory Gi/o subunit GOA-1 regulates spermathecal transits. We employed TurboID proximity labeling and mass spectrometry to identify 16 candidate interactors of GOA-1. Depletion of these candidates by RNAi did not yield overt spermathecal transit defects.

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Sadeghian, F., Cram, E. J.. 2025-10-02. GOA-1 regulates spermathecal transits. https://doi.org/10.1101/2025.09.30.679514

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