Local thalamic interneurons drive spindle termination and enable sleep-dependent learning

Abstract

The thalamus is central to fundamental brain functions including sensation, attention, and sleep through the precise generation and regulation of neuronal ensemble oscillatory activity. Sensory thalamic circuits are considered feedforward structures, lacking lateral connectivity, while recurrence in the network is mediated by interactions with inhibitory neurons of the thalamic reticular nucleus. Here, we define previously uncharacterized functional roles of local thalamic interneurons, a component of the sensory thalamus whose function has remained unexplored. We demonstrate that local interneuron activation induces rebound oscillations in thalamocortical relay neurons ex vivo and neocortical spindles in vivo, while their inhibition increases spindle occurrence, overall spindle duration and impairs sensory learning. Our findings reveal that local thalamic interneurons have shared and complementary functions to those of thalamic reticular neurons and are required for proper spindle formation and sleep-dependent learning. Together, this work establishes an important neural substrate of thalamocortical circuit function.